Bacteriocin Production by Halophilic and Halotolerant Bacteria from Lake Chairkota, Potosí Bolivia
DOI:
https://doi.org/10.52428/20756208.v12i29.518Keywords:
Halophilic bacteria, Bacteriocins, Staphylococcus equorum, Bolivian AltiplanoAbstract
En las últimas décadas se ha estudiado extensamente la capacidad de las bacterias halófilas de producir sustancias de interés biotecnológico. El Centro de Biotecnología de la Universidad Mayor de San Simón cuenta con un banco de microorganismos halófilos aislados del altiplano boliviano, de los cuales se seleccionó 71 cepas halófilas y halo tolerantes de la Laguna Chairkota para analizar su capacidad de producción de sustancias antibióticas o bacteriocinas. Se realizó un screening de las cepas en medio de cultivo sólido, utilizando la técnica de la doble capa, y se encontró 14 cepas capaces de producir bacteriocinas. Posteriormente se seleccionó a la cepa halo tolerante CH20 como mejor productora que al ser secuenciada, analizada filogenéticamente y caracterizada morfológica y bioquímicamente. Se encontró que pertenece a la especie Staphy/ococcus equorum, especie descrita en bibliografía, aislada de medios con alto contenido de sal. Se denominó a la cepa identificada como Staphylococcus equorum CH20. Esta investigación demuestra la capacidad biotecnológica de los microorganismos halófilos. Especialmente el potencial que tienen las cepas aisladas del altiplano boliviano, las cuales pueden ser utilizadas en un futuro como alternativa a los antibióticos comúnmente comercializados.
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Copyright (c) 2017 Paola V. Ayala Borda, Virginia A. Vargas Calle y Jerry L. Solis Valdivia
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